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Standards for Cell Line Authentication and Beyond.

ABSTRACT: Different genomic technologies have been applied to cell line authentication, but only one method (short tandem repeat [STR] profiling) has been the subject of a comprehensive and definitive standard (ASN-0002). Here we discuss the power of this document and why standards such as this are so critical for establishing the consensus technical criteria and practices that can enable progress in the fields of research that use cell lines. We also examine other methods that could be used for authentication and discuss how a combination of methods could be used in a holistic fashion to assess various critical aspects of the quality of cell lines.

SUBMITTER: Almeida JL

PROVIDER: S-EPMC4907466 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

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Standards for Cell Line Authentication and Beyond.

Almeida Jamie L JL Cole Kenneth D KD Plant Anne L AL

PLoS biology 20160614 6

Different genomic technologies have been applied to cell line authentication, but only one method (short tandem repeat [STR] profiling) has been the subject of a comprehensive and definitive standard (ASN-0002). Here we discuss the power of this document and why standards such as this are so critical for establishing the consensus technical criteria and practices that can enable progress in the fields of research that use cell lines. We also examine other methods that could be used for authentic ...[more]

PMID: 27300367

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Author Correction: Authentication and characterisation of a new oesophageal adenocarcinoma cell line: MFD-1.

Project description:A correction has been published and is appended to both the HTML and PDF versions of this paper. The error has not been fixed in the paper.

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