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Plant-specific DUF1110 protein from Oryza sativa: expression, purification and crystallization.

ABSTRACT: The Os01T0156300 protein from Oryza sativa has been classified into the domain of unknown function (DUF) family DUF1110. DUF1110 family members exist in monocotyledons but not in dicotyledons, and share no sequence identity with proteins for which structures have been reported. In this study, the Os01T0156300 protein was crystallized using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 1.84?Å resolution. The crystal belonged to space group P21, with unit-cell parameters a = 89.9, b = 89.8, c = 107.1?Å, ? = 106.6°. The asymmetric unit was estimated to contain 6-11 molecules.

SUBMITTER: Harada K

PROVIDER: S-EPMC4909249 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

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Plant-specific DUF1110 protein from Oryza sativa: expression, purification and crystallization.

Harada Kenichi K Yamashita Eiki E Inoue Kento K Yamaguchi Koji K Fujiwara Toshimichi T Nakagawa Atsushi A Kawasaki Tsutomu T Kojima Chojiro C

Acta crystallographica. Section F, Structural biology communications 20160523 Pt 6

The Os01T0156300 protein from Oryza sativa has been classified into the domain of unknown function (DUF) family DUF1110. DUF1110 family members exist in monocotyledons but not in dicotyledons, and share no sequence identity with proteins for which structures have been reported. In this study, the Os01T0156300 protein was crystallized using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 1.84 Å resolution. The crystal belonged to space group P21, with unit-cell ...[more]

PMID: 27303902

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