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Chemically activatable viral capsid functionalized for cancer targeting.


ABSTRACT: To design a theranostic capsule using the virus-like nanoparticle of the hepatitis E virus modified to display breast cancer cell targeting functional group (LXY30).Five surface-exposed residues were mutated to cysteine to allow conjugation to maleimide-linked chemical groups via thiol-selective linkages. Engineered virus-like nanoparticles were then covalently conjugated to a breast cancer recognized ligand, LXY30 and an amine-coupled near-infrared fluorescence dye.LXY30-HEV VLP was checked for its binding and entry to a breast cancer cell line and for tumor targeting in vivo to breast cancer tissue in mice. The engineered virus-like nanoparticle not only targeted cancer cells, but also appeared immune silent to native hepatitis E virus antibodies due to epitope disruption at the antibody-binding site.These results demonstrate the production of a theranostic capsule suitable for cancer diagnostics and therapeutics based on surface modification of a highly stable virus-like nanoparticle.

SUBMITTER: Chen CC 

PROVIDER: S-EPMC4910953 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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Chemically activatable viral capsid functionalized for cancer targeting.

Chen Chun-Chieh CC   Xing Li L   Stark Marie M   Ou Tingwei T   Holla Prasida P   Xiao Kai K   Kamita Shizuo G SG   Hammock Bruce D BD   Lam Kit K   Cheng R Holland RH  

Nanomedicine (London, England) 20160120 4


<h4>Aim</h4>To design a theranostic capsule using the virus-like nanoparticle of the hepatitis E virus modified to display breast cancer cell targeting functional group (LXY30).<h4>Methods</h4>Five surface-exposed residues were mutated to cysteine to allow conjugation to maleimide-linked chemical groups via thiol-selective linkages. Engineered virus-like nanoparticles were then covalently conjugated to a breast cancer recognized ligand, LXY30 and an amine-coupled near-infrared fluorescence dye.<  ...[more]

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