Lipid Discovery by Combinatorial Screening and Untargeted LC-MS/MS.
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ABSTRACT:
SUBMITTER: Bilgin M
PROVIDER: S-EPMC4911551 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
Publications
Lipid Discovery by Combinatorial Screening and Untargeted LC-MS/MS.
Bilgin Mesut M Born Petra P Fezza Filomena F Heimes Michael M Mastrangelo Nicolina N Wagner Nicolai N Schultz Carsten C Maccarrone Mauro M Eaton Suzanne S Nadler André A Wilm Matthias M Shevchenko Andrej A
Scientific reports 20160617
We present a method for the systematic identification of picogram quantities of new lipids in total extracts of tissues and fluids. It relies on the modularity of lipid structures and applies all-ions fragmentation LC-MS/MS and Arcadiate software to recognize individual modules originating from the same lipid precursor of known or assumed structure. In this way it alleviates the need to recognize and fragment very low abundant precursors of novel molecules in complex lipid extracts. In a single ...[more]
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