Ontology highlight
ABSTRACT:
SUBMITTER: Feng J
PROVIDER: S-EPMC4911744 | biostudies-literature | 2016 Jul
REPOSITORIES: biostudies-literature
Feng Jianxun J Gan Haiyun H Eaton Matthew L ML Zhou Hui H Li Shuqi S Belsky Jason A JA MacAlpine David M DM Zhang Zhiguo Z Li Qing Q
Molecular and cellular biology 20160615 13
FACT (facilitates chromatin transcription) consists of two essential subunits, Spt16 and Pob3, and functions as a histone chaperone. Mutation of spt16 results in a global loss of nucleosomes as well as aberrant transcription. Here, we show that the majority of nucleosome changes upon Spt16 depletion are alterations in nucleosome fuzziness and position shift. Most nucleosomal changes are suppressed by the inhibition of RNA polymerase II (Pol II) activity. Surprisingly, a small subgroup of nucleos ...[more]