Ontology highlight
ABSTRACT:
SUBMITTER: Jacob T
PROVIDER: S-EPMC4913309 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
Jacob Thomas T Agarwal Anupriya A Ramunno-Johnson Damien D O'Hare Thomas T Gönen Mehmet M Tyner Jeffrey W JW Druker Brian J BJ Vu Tania Q TQ
Scientific reports 20160620
Many important signaling and regulatory proteins are expressed at low abundance and are difficult to measure in single cells. We report a molecular imaging approach to quantitate protein levels by digitized, discrete counting of nanoparticle-tagged proteins. Digitized protein counting provides ultrasensitive molecular detection of proteins in single cells that surpasses conventional methods of quantitating total diffuse fluorescence, and offers a substantial improvement in protein quantitation. ...[more]