Universal multiplexable matK primers for DNA barcoding of angiosperms.
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ABSTRACT:
SUBMITTER: Heckenhauer J
PROVIDER: S-EPMC4915916 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
Publications
Universal multiplexable matK primers for DNA barcoding of angiosperms.
Heckenhauer Jacqueline J Barfuss Michael H J MH Samuel Rosabelle R
Applications in plant sciences 20160608 6
<h4>Premise of the study</h4>PCR amplification of the matK barcoding region is often difficult when dealing with multiple angiosperm families. We developed a primer cocktail to amplify this region efficiently across angiosperm diversity.<h4>Methods and results</h4>We developed 14 matK primers (seven forward, seven reverse) for multiplex PCR, using sequences available in GenBank for 178 taxa belonging to 123 genera in 41 families and 18 orders. Universality of these new multiplexed primers was te ...[more]
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