Ontology highlight
ABSTRACT:
SUBMITTER: Rajasekaran B
PROVIDER: S-EPMC4917178 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
PloS one 20160622 6
Many questions in developmental biology depend on measuring the position and movement of individual cells within developing embryos. Yet, tools that provide this data are often challenged by high cell density and their accuracy is difficult to measure. Here, we present a three-step procedure to address this problem. Step one is a novel segmentation algorithm based on image derivatives that, in combination with selective post-processing, reliably and automatically segments cell nuclei from images ...[more]