Dataset Information

Invasion Fosters Change: Independent Evolutionary Shifts in Reproductive Traits after Oxalis pes-caprae L. Introduction.

ABSTRACT: Biological invasions offer optimal scenarios to study evolutionary changes under contemporary timescales. After long-distance dispersal, exotic species have to cope with strong mate limitation, and shifts toward uniparental reproduction have been hypothesized to be selectively advantageous. Oxalis pes-caprae is a clonal tristylous species native to South Africa, and invasive in Mediterranean regions worldwide. It reproduces sexually and asexually but the importance of each strategy differs between ranges. Native populations reproduce mostly sexually while in invasive ones asexual reproduction is the prevailing strategy due to the dominance of pentaploid monomorphic populations. Nevertheless, two contrasting scenarios have been observed after introduction: transition toward clonality, and re-acquisition of sexuality fueled by multiple introductions of compatible mates. Here, we aimed to assess evolutionary changes of reproductive traits in O. pes-caprae invasive populations and evaluate whether these traits could be related with invasion success and prevalence of certain forms in the western Mediterranean basin. Sexual and asexual reproduction traits were quantified under optimal conditions in a common garden experiment including native and invasive sexual, predominately asexual, and obligated asexual individuals. Different reproductive, ecological, and genetic constraints created by long-distance dispersal seem to have generated different selective pressures in sexual and asexual traits, with our results supporting evolutionary changes in invasive populations of O. pes-caprae. Native plants had higher sexual fitness, while a transition toward clonality was clear for invasive forms, supporting clonal reproduction as a major trait driving invasion. Differences were also observed among invasive plants, with sexual forms having increased dispersal potential; thus, they are expected to be in advantage in comparison with predominantly asexual and obligated asexual plants, and may become widespread in the future. Historical processes, like the initial introduction of predominantly asexual forms followed by sexual forms more recently, could be in the origin of current distribution patterns of O. pes-caprae in the western Mediterranean. This study shows that invasion processes are very dynamic and that ecological and genetic constraints determined by the invasion process may originate different reproductive strategies that are likely to determine invasion success.

SUBMITTER: Castro S

PROVIDER: S-EPMC4919335 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Invasion Fosters Change: Independent Evolutionary Shifts in Reproductive Traits after Oxalis pes-caprae L. Introduction.

Castro Sílvia S Castro Mariana M Ferrero Victoria V Costa Joana J Tavares Daniela D Navarro Luis L Loureiro João J

Frontiers in plant science 20160624

Biological invasions offer optimal scenarios to study evolutionary changes under contemporary timescales. After long-distance dispersal, exotic species have to cope with strong mate limitation, and shifts toward uniparental reproduction have been hypothesized to be selectively advantageous. Oxalis pes-caprae is a clonal tristylous species native to South Africa, and invasive in Mediterranean regions worldwide. It reproduces sexually and asexually but the importance of each strategy differs betwe ...[more]

PMID: 27446109

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Project description:Oxalis pes-caprae L. is a South African geophyte that behaves as an invasive in the eurimediterranean area. According to a long-established hypothesis, O. pes-caprae may have invaded Europe and the Mediterranean area starting from a single plant introduced in the Botanical Garden of Malta at the beginning of the 19th century. The aim of this work was to test this hypothesis, to track the arrival of O. pes-caprae in different countries of the Euro-Mediterranean area and to understand the pathways of spreading and particularly its starting point(s). Historical data attesting the presence of the plant in the whole Euro-Mediterranean region were collected from different sources: herbarium specimens, Floras and other botanical papers, plant lists of gardens, catalogs of plant nurseries and plant dealers. First records of the plant (both cultivated and wild) for each Territorial Unit (3rd level of NUTS) were selected and used to draw up a diachronic map and an animated graphic. Both documents clearly show that oldest records are scattered throughout the whole area, proving that the plant arrived in Europe and in the Mediterranean region more times independently and that its spreading started in different times from several different centers of invasion. Botanical gardens and other public or private gardens, nurseries and plant dealers, and above all seaside towns and harbors seemingly played a strategic role as a source of either intentional and unintentional introduction or spread. A geographic profiling analysis was performed to analyse the data. We used also techniques (Silhouette, Kmeans and Voronoi tessellation) capable of verifying the presence of more than one independent clusters of data on the basis of their geographical distribution. Microsatellites were employed for a preliminary analysis of genetic variation in the Mediterranean. Even if the sampling was insufficient, particularly among the populations of the original area, our data supported three main groups of populations, one of them corresponding to the central group of populations identified by GP analysis, and the other two corresponding, respectively, to the western and the eastern cluster of data. The most probable areas of origin of the invasion in the three clusters of observations are characterized by the presence of localities where the invasive plant was cultivated, with the exception of the Iberian cluster of observation where the observations in the field predate the data about known cultivation localities. Alternative possible reasons are also suggested, to explain the current prevalence of pentaploid short-styled plants in the Euro-Mediterranean area.

Project description:Phytochemicals are directly involved in therapeutic treatment or precursors to synthesize useful drugs. The current study was aimed to evaluate the phytocompounds and their biopotentials using methanolic and n-hexane extracts of various parts of Oxalis pes-caprae. For the phytochemical analysis, standard procedures were used, whereas Aluminum Chloride reagent and Follin-ciocalteau reagent methods were used to determine total flavonoid and phenolic contents. Radical scavenging DPPH, phosphomolybdenum reduction, and reducing power assays were used to assess antioxidative potentials. Antibacterial potential was determined by applying disc diffusion method while cytotoxicity was determined employing brine shrimp assay. FT-IR (Fourier-transform infrared) analysis was utilized to gather spectral information, while molecular docking tools were employed to look at how O. pes-caprae plant-based ligands interact with the target protein COVID-19 3CLPro (PDB:6LU7). Phenols, flavonoids, alkaloids and saponins were tested positive in preliminary phytochemical studies. TPC and TFC in different extracts ranging from (38.55 ± 1.72) to (65.68 ± 0.88) mg/g GAE/g and (24.75 ± 1.80) to (14.83 ± 0.92) mg/g QUE/g were used respectively. IC50 value (24.75 ± 0.76 g/mL) by OXFH, total antioxidant capacity (55.89 ± 1.75) mg/g by OXLM, reducing potential (34.98 ± 1.089) mg/g by OXSM, maximum zone of inhibition against B. subtilis (24 ± 0.65 mm) by OXLM and maximum cytotoxicity 96% with LD50 19.66 (μg/mL) by OXSM were the best calculated values among all extracts. Using molecular docking, it was found that Caeruleanone A, 2',4'-Dihydroxy-2″-(1-hydroxy-1-methylethyl) dihydrofuro [2,3-h] flavanone and Vadimezan demonstrated best affinity with the investigated SARS CoV-2 Mpro protein. This work provide justification about this plant as a source of effective phytochemicals and their potential against microbes could lead to development of biosafe drugs for the welfare of human being. In future, different in vitro and in vivo biological studies can be performed to further investigate its biomedical potentials.

Project description:Ipomoea pes-caprae (Convolvulaceae), a pantropical plant with sea-drifted seeds, is found globally in the littoral areas of tropical and subtropical regions. Unusual long-distance seed dispersal has been believed to be responsible for its extraordinarily wide distribution; however, the actual level of inter-population migration has never been studied. To clarify the level of migration among populations of I. pes-caprae across its range, we investigated nucleotide sequence variations by using seven low-copy nuclear markers and 272 samples collected from 34 populations that cover the range of the species. We applied coalescent-based approaches using Bayesian and maximum likelihood methods to assess migration rates, direction of migration, and genetic diversity among five regional populations. Our results showed a high number of migrants among the regional populations of I. pes-caprae subsp. brasiliensis, which suggests that migration among distant populations was maintained by long-distance seed dispersal across its global range. These results also provide strong evidence for recent trans-oceanic seed dispersal by ocean currents in all three oceanic regions. We also found migration crossing the American continents. Although this is an apparent land barrier for sea-dispersal, migration between populations of the East Pacific and West Atlantic regions was high, perhaps because of trans-isthmus migration via pollen dispersal. Therefore, the migration and gene flow among populations across the vast range of I. pes-caprae is maintained not only by seed dispersal by sea-drifted seeds, but also by pollen flow over the American continents. On the other hand, populations of subsp. pes-caprae that are restricted to only the northern part of the Indian Ocean region were highly differentiated from subsp. brasiliensis. Cryptic barriers that prevented migration by sea dispersal between the ranges of the two subspecies and/or historical differentiation that caused local adaptation to different environmental factors in each region could explain the genetic differentiation between the subspecies.

Dataset Information

Invasion Fosters Change: Independent Evolutionary Shifts in Reproductive Traits after Oxalis pes-caprae L. Introduction.

Publications

Invasion Fosters Change: Independent Evolutionary Shifts in Reproductive Traits after Oxalis pes-caprae L. Introduction.

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