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Plant growth-promoting actinobacteria on chickpea seed mineral density: an upcoming complementary tool for sustainable biofortification strategy.

ABSTRACT: The present study was evaluated to test the potential of plant growth-promoting actinobacteria in increasing seed mineral density of chickpea under field conditions. Among the 19 isolates of actinobacteria tested, significant (p < 0.05) increase of minerals over the uninoculated control treatments was noticed on all the isolates for Fe (10-38 %), 17 for Zn (13-30 %), 16 for Ca (14-26 %), 9 for Cu (11-54 %) and 10 for Mn (18-35 %) and Mg (14-21 %). The increase might be due to the production of siderophore-producing capacity of the tested actinobacteria, which was confirmed in our previous studies by q-RT PCR on siderophore genes expressing up to 1.4- to 25-fold increased relative transcription levels. The chickpea seeds were subjected to processing to increase the mineral availability during consumption. The processed seeds were found to meet the recommended daily intake of FDA by 24-28 % for Fe, 25-28 % for Zn, 28-35 % for Cu, 12-14 % for Ca, 160-167 % for Mn and 34-37 % for Mg. It is suggested that the microbial inoculum can serve as a complementary sustainable tool for the existing biofortification strategies and substantially reduce the chemical fertilizer inputs.

SUBMITTER: Sathya A

PROVIDER: S-EPMC4919949 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Plant growth-promoting actinobacteria on chickpea seed mineral density: an upcoming complementary tool for sustainable biofortification strategy.

Sathya Arumugam A Vijayabharathi Rajendran R Srinivas Vadlamudi V Gopalakrishnan Subramaniam S

3 Biotech 20160621 2

The present study was evaluated to test the potential of plant growth-promoting actinobacteria in increasing seed mineral density of chickpea under field conditions. Among the 19 isolates of actinobacteria tested, significant (p < 0.05) increase of minerals over the uninoculated control treatments was noticed on all the isolates for Fe (10-38 %), 17 for Zn (13-30 %), 16 for Ca (14-26 %), 9 for Cu (11-54 %) and 10 for Mn (18-35 %) and Mg (14-21 %). The increase might be due to the production of s ...[more]

PMID: 28330210

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Project description:Introduction and aimsThe intensive cropping system and imbalance use of chemical fertilizers to pursue high grain production and feed the fast-growing global population has disturbed agricultural sustainability and nutritional security. Understanding micronutrient fertilizer management especially zinc (Zn) through foliar application is a crucial agronomic approach that could improve agronomic biofortification of staple grain crops. The use of plant growth-promoting bacteria (PGPBs) is considered as one of the sustainable and safe strategies that could improve nutrient acquisition and uptake in edible tissues of wheat to combat Zn malnutrition and hidden hunger in humans. Therefore, the objective of this study was to evaluate the best-performing PGPB inoculants in combination with nano-Zn foliar application on the growth, grain yield, and concentration of Zn in shoots and grains, Zn use efficiencies, and estimated Zn intake under wheat cultivation in the tropical savannah of Brazil.MethodsThe treatments consisted of four PGPB inoculations (without inoculation, Azospirillum brasilense, Bacillus subtilis, and Pseudomonas fluorescens, applied by seeds) and five Zn doses (0, 0.75, 1.5, 3, and 6 kg ha-1, applied from nano ZnO in two splits by leaf).ResultsInoculation of B. subtilis and P. fluorescens in combination with 1.5 kg ha-1 foliar nano-Zn fertilization increased the concentration of Zn, nitrogen, and phosphorus in the shoot and grain of wheat in the 2019 and 2020 cropping seasons. Shoot dry matter was increased by 5.3% and 5.4% with the inoculation of P. fluorescens, which was statistically not different from the treatments with inoculation of B. subtilis as compared to control. The grain yield of wheat was increased with increasing nano-Zn foliar application up to 5 kg Zn ha-1 with the inoculation of A. brasilense in 2019, and foliar nano-Zn up to a dose of 1.5 kg ha-1 along with the inoculation of P. fluorescens in the 2020 cropping season. The zinc partitioning index was increased with increasing nano Zn application up to 3 kg ha-1 along with the inoculation of P. fluorescens. Zinc use efficiency and applied Zn recovery were improved at low doses of nano-Zn application in combination with the inoculation of A. brasilense, B. subtilis, and P. fluorescens, respectively, as compared to control.DiscussionTherefore, inoculation with B. subtilis and P. fluorescens along with foliar nano-Zn application is considered a sustainable and environmentally safe strategy to increase nutrition, growth, productivity, and Zn biofortification of wheat in tropical savannah.

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Plant growth-promoting actinobacteria on chickpea seed mineral density: an upcoming complementary tool for sustainable biofortification strategy.

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Plant growth-promoting actinobacteria on chickpea seed mineral density: an upcoming complementary tool for sustainable biofortification strategy.

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