Guidelines for the optimal design of miRNA-based shRNAs.
Ontology highlight
ABSTRACT:
SUBMITTER: Bofill-De Ros X
PROVIDER: S-EPMC4921303 | biostudies-literature | 2016 Jul
REPOSITORIES: biostudies-literature
Publications
Guidelines for the optimal design of miRNA-based shRNAs.
Bofill-De Ros Xavier X Gu Shuo S
Methods (San Diego, Calif.) 20160412
RNA interference (RNAi) is an extremely useful tool for inhibiting gene expression. It can be triggered by transfected synthetic small interfering RNA (siRNA) or by expressed small hairpin RNA (shRNA). The cellular machinery processes the latter into siRNA in vivo. shRNA is preferred or required in genetic screens and specific RNAi approaches in gene therapy settings. Despite its many successes, the field of shRNAs faces many challenges. Insufficient knockdowns and off-target effects become obst ...[more]
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