Ontology highlight
ABSTRACT:
SUBMITTER: Iglesias-Figueroa B
PROVIDER: S-EPMC4926436 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
Iglesias-Figueroa Blanca B Valdiviezo-Godina Norberto N Siqueiros-Cendón Tania T Sinagawa-García Sugey S Arévalo-Gallegos Sigifredo S Rascón-Cruz Quintín Q
International journal of molecular sciences 20160609 6
In this study, bovine lactoferrin (bLf), an iron-binding glycoprotein considered an important nutraceutical protein because of its several properties, was expressed in Pichia pastoris KM71-H under AOX1 promoter control, using pJ902 as the recombinant plasmid. Dot blotting analysis revealed the expression of recombinant bovine lactoferrin (rbLf) in Pichia pastoris. After Bach fermentation and purification by molecular exclusion, we obtained an expression yield of 3.5 g/L of rbLf. rbLf and predomi ...[more]