PCR primers for 30 novel gene regions in the nuclear genomes of Lepidoptera.
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ABSTRACT:
SUBMITTER: Wahlberg N
PROVIDER: S-EPMC4926658 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Publications
PCR primers for 30 novel gene regions in the nuclear genomes of Lepidoptera.
Wahlberg Niklas N Peña Carlos C Ahola Milla M Wheat Christopher W CW Rota Jadranka J
ZooKeys 20160608 596
We report primer pairs for 30 new gene regions in the nuclear genomes of Lepidoptera that can be amplified using a standard PCR protocol. The new primers were tested across diverse Lepidoptera, including nonditrysians and a wide selection of ditrysians. These new gene regions give a total of 11,043 bp of DNA sequence data and they show similar variability to traditionally used nuclear gene regions in studies of Lepidoptera. We feel that a PCR-based approach still has its place in molecular syste ...[more]
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