Dataset Information

The Expression of Inflammatory Mediators in Bladder Pain Syndrome.

ABSTRACT:

Background

Bladder pain syndrome (BPS) pathology is poorly understood. Treatment strategies are empirical, with limited efficacy, and affected patients have diminished quality of life.

Objective

We examined the hypothesis that inflammatory mediators within the bladder contribute to BPS pathology.

Design, setting, and participants

Fifteen women with BPS and 15 women with stress urinary incontinence without bladder pain were recruited from Cork University Maternity Hospital from October 2011 to October 2012. During cystoscopy, 5-mm bladder biopsies were taken and processed for gene expression analysis. The effect of the identified genes was tested in laboratory animals.

Outcome measures and statistical analysis

We studied the expression of 96 inflammation-related genes in diseased and healthy bladders. We measured the correlation between genes and patient clinical profiles using the Pearson correlation coefficient.

Results and limitations

Analysis revealed 15 differentially expressed genes, confirmed in a replication study. FGF7 and CCL21 correlated significantly with clinical outcomes. Intravesical CCL21 instillation in rats caused increased bladder excitability and increased c-fos activity in spinal cord neurons. CCL21 atypical receptor knockout mice showed significantly more c-fos upon bladder stimulation with CCL21 than wild-type littermates. There was no change in FGF7-treated animals. The variability in patient samples presented as the main limitation. We used principal component analysis to identify similarities within the patient group.

Conclusions

Our study identified two biologically relevant inflammatory mediators in BPS and demonstrated an increase in nociceptive signalling with CCL21. Manipulation of this ligand is a potential new therapeutic strategy for BPS.

Patient summary

We compared gene expression in bladder biopsies of patients with bladder pain syndrome (BPS) and controls without pain and identified two genes that were increased in BPS patients and correlated with clinical profiles. We tested the effect of these genes in laboratory animals, confirming their role in bladder pain. Manipulating these genes in BPS is a potential treatment strategy.

SUBMITTER: Offiah I

PROVIDER: S-EPMC4926725 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature

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Publications

The Expression of Inflammatory Mediators in Bladder Pain Syndrome.

Offiah Ifeoma I Didangelos Athanasios A Dawes John J Cartwright Rufus R Khullar Vik V Bradbury Elizabeth J EJ O'Sullivan Suzanne S Williams Dic D Chessell Iain P IP Pallas Kenny K Graham Gerry G O'Reilly Barry A BA McMahon Stephen B SB

European urology 20160307 2

<h4>Background</h4>Bladder pain syndrome (BPS) pathology is poorly understood. Treatment strategies are empirical, with limited efficacy, and affected patients have diminished quality of life.<h4>Objective</h4>We examined the hypothesis that inflammatory mediators within the bladder contribute to BPS pathology.<h4>Design, setting, and participants</h4>Fifteen women with BPS and 15 women with stress urinary incontinence without bladder pain were recruited from Cork University Maternity Hospital f ...[more]

PMID: 26965559

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Dataset Information

The Expression of Inflammatory Mediators in Bladder Pain Syndrome.

Background

Objective

Design, setting, and participants

Outcome measures and statistical analysis

Results and limitations

Conclusions

Patient summary

Publications

The Expression of Inflammatory Mediators in Bladder Pain Syndrome.

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