?B-crystallin is essential for the TGF-?2-mediated epithelial to mesenchymal transition of lens epithelial cells.

ABSTRACT: Transforming growth factor (TGF)-?2-mediated pathways play a major role in the epithelial to mesenchymal transition (EMT) of lens epithelial cells (LECs) during secondary cataract formation, which is also known as posterior capsule opacification (PCO). Although ?B-crystallin is a major protein in LEC, its role in the EMT remains unknown. In a human LEC line (FHL124), TGF-?2 treatment resulted in changes in the EMT-associated proteins at the mRNA and protein levels. This was associated with nuclear localization of ?B-crystallin, phosphorylated Smad2 (pSmad2) (S245/250/255), pSmad3 (S423/425), Smad4 and Snail and the binding of ?B-crystallin to these transcription factors, all of which were reduced by the down-regulation of ?B-crystallin. Expression of the functionally defective R120G mutant of ?B-crystallin reduced TGF-?2-induced EMT in LECs of ?B-crystallin knockout (KO) mice. Treatment of bovine lens epithelial explants and mouse LEC with TGF-?2 resulted in changes in the EMT-associated proteins at the mRNA and protein levels. This was accompanied by increase in phosphorylation of p44/42 mitogen-activated protein kinases (MAPK) (T202/Y204), p38 MAPK (T180/Y182), protein kinase B (Akt) (S473) and Smad2 when compared with untreated cells. These changes were significantly reduced in ?B-crystallin depleted or knocked out LEC. The removal of the fibre cell mass from the lens of wild-type (WT) mice resulted in the up-regulation of EMT-associated genes in the capsule-adherent epithelial cells, which was reduced in the ?B-crystallin KO mice. Together, our data show that ?B-crystallin plays a central role in the TGF-?2-induced EMT of LEC. ?B-Crystallin could be targeted to prevent PCO and pathological fibrosis in other tissues.

SUBMITTER: Nahomi RB 

PROVIDER: S-EPMC4928857 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature