Ontology highlight
ABSTRACT:
SUBMITTER: Veerareddygari GR
PROVIDER: S-EPMC4929527 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
Veerareddygari Govardhan Reddy GR Singh Sanjay K SK Mueller Eugene G EG
Journal of the American Chemical Society 20160617 25
The pseudouridine synthases isomerize (U) in RNA to pseudouridine (Ψ), and the mechanism that they follow has long been a question of interest. The recent elucidation of a product of the mechanistic probe 5-fluorouridine that had been epimerized to the arabino isomer suggested that the Ψ synthases might operate through a glycal intermediate formed by deprotonation of C2'. When that position in substrate U is deuterated, a primary kinetic isotope effect is observed, which indisputably indicates t ...[more]