Volumetric Light-Field Excitation.

ABSTRACT: We explain how to concentrate light simultaneously at multiple selected volumetric positions by means of a 4D illumination light field. First, to select target objects, a 4D imaging light field is captured. A light field mask is then computed automatically for this selection to avoid illumination of the remaining areas. With one-photon illumination, simultaneous generation of complex volumetric light patterns becomes possible. As a full light-field can be captured and projected simultaneously at the desired exposure and excitation times, short readout and lighting durations are supported.

SUBMITTER: Schedl DC 

PROVIDER: S-EPMC4929678 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature