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Rapid high mass resolution mass spectrometry using matrix-assisted ionization.

ABSTRACT: Matrix-assisted ionization (MAI) is demonstrated to be a robust and sensitive analytical method capable of analyzing proteins such as cholera toxin B-subunit and pertussis toxin mutant from conditions containing relatively high amounts of inorganic salts, buffers, and preservatives without the need for prior sample clean-up or concentration. By circumventing some of the sample preparation steps, MAI simplifies and accelerates the analytical workflow for biological samples in complex media. The benefits of multiply charged ions characteristic of electrospray ionization (ESI) and the robustness of matrix-assisted laser desorption/ionization (MALDI) can be obtained from a single method, making it well suited for analysis of proteins and other biomolecules at ultra-high resolution as demonstrated on an Orbitrap Fusion where protein subunits were resolved for which MALDI-time-of-flight failed. MAI results are compared with those obtained with ESI, MALDI, and laserspray ionization methods and fundamental commonalities discussed.

SUBMITTER: Trimpin S 

PROVIDER: S-EPMC4930895 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

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Rapid high mass resolution mass spectrometry using matrix-assisted ionization.

Trimpin Sarah S   Thawoos Shameemah S   Foley Casey D CD   Woodall Daniel W DW   Li Jing J   Inutan Ellen D ED   Stemmer Paul M PM  

Methods (San Diego, Calif.) 20160204

Matrix-assisted ionization (MAI) is demonstrated to be a robust and sensitive analytical method capable of analyzing proteins such as cholera toxin B-subunit and pertussis toxin mutant from conditions containing relatively high amounts of inorganic salts, buffers, and preservatives without the need for prior sample clean-up or concentration. By circumventing some of the sample preparation steps, MAI simplifies and accelerates the analytical workflow for biological samples in complex media. The b  ...[more]

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