Programming a topologically constrained DNA nanostructure into a sensor.

ABSTRACT: Many rationally engineered DNA nanostructures use mechanically interlocked topologies to connect individual DNA components, and their physical connectivity is achieved through the formation of a strong linking duplex. The existence of such a structural element also poses a significant topological constraint on functions of component rings. Herein, we hypothesize and confirm that DNA catenanes with a strong linking duplex prevent component rings from acting as the template for rolling circle amplification (RCA). However, by using an RNA-containing DNA [2] catenane with a strong linking duplex, we show that a stimuli-responsive RNA-cleaving DNAzyme can linearize one component ring, and thus enable RCA, producing an ultra-sensitive biosensing system. As an example, a DNA catenane biosensor is engineered to detect the model bacterial pathogen Escherichia coli through binding of a secreted protein, with a detection limit of 10?cells?ml(-1), thus establishing a new platform for further applications of mechanically interlocked DNA nanostructures.

SUBMITTER: Liu M 

PROVIDER: S-EPMC4931013 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature