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Mitotic genes are transcriptionally upregulated in the fibroblast irradiated with very low doses of UV-C.

ABSTRACT: Ultraviolet (UV) radiation induces a variety of biological effects, including DNA damage response and cell signaling pathways. We performed transcriptome analysis using microarray in human primary cultured fibroblasts irradiated with UV-C (0.5 or 5?J/m(2)) and harvested at 4 or 12?h following UV exposure. All transcript data were analyzed by comparison with the corresponding results in non-irradiated (control) cells. The number of genes with significantly altered expression (?2-fold difference relative to the control) is higher in the sample irradiated with high dose of UV, suggesting that gene expression was UV dose-dependent. Pathway analysis on the upregulated genes at 12?h indicates that the expression of some cell cycle-related genes was predominantly induced irrespective of UV-dose. Interestingly, almost all the genes with significant altered expression were cell cycle-related genes designated as 'Mitotic Genes', which function in the spindle assembly checkpoint. Therefore, even a low dose of UV could affect the transcriptional profile.

SUBMITTER: Takeuchi S

PROVIDER: S-EPMC4932599 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

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Mitotic genes are transcriptionally upregulated in the fibroblast irradiated with very low doses of UV-C.

Takeuchi Seiji S Matsuda Toshiro T Ono Ryusuke R Tsujimoto Mariko M Nishigori Chikako C

Scientific reports 20160705

Ultraviolet (UV) radiation induces a variety of biological effects, including DNA damage response and cell signaling pathways. We performed transcriptome analysis using microarray in human primary cultured fibroblasts irradiated with UV-C (0.5 or 5 J/m(2)) and harvested at 4 or 12 h following UV exposure. All transcript data were analyzed by comparison with the corresponding results in non-irradiated (control) cells. The number of genes with significantly altered expression (≥2-fold difference r ...[more]

PMID: 27378355

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Dataset Information

Mitotic genes are transcriptionally upregulated in the fibroblast irradiated with very low doses of UV-C.

Publications

Mitotic genes are transcriptionally upregulated in the fibroblast irradiated with very low doses of UV-C.

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