Ontology highlight
ABSTRACT:
SUBMITTER: Kim HM
PROVIDER: S-EPMC4932911 | biostudies-literature | 2016 Jul
REPOSITORIES: biostudies-literature
Kim Hyun-Min HM Colaiácovo Monica P MP
Current protocols in molecular biology 20160701
The CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) system is successfully being used for efficient and targeted genome editing in various organisms, including the nematode C. elegans. Recent studies have developed various CRISPR-Cas9 approaches to enhance genome engineering via two major DNA double-strand break repair pathways: non-homologous end joining and homologous recombination. Here we describe a protocol for Cas9-mediated C. elegans genome editi ...[more]