Ontology highlight
ABSTRACT:
SUBMITTER: Vantangoli MM
PROVIDER: S-EPMC4933361 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Vantangoli Marguerite M MM Madnick Samantha J SJ Wilson Shelby S Boekelheide Kim K
PloS one 20160705 7
The development of three-dimensional (3D) cultures is increasing, as they are able to provide the utility of in vitro models and the strength of testing in physiologically relevant systems. When cultured in a scaffold-free agarose hydrogel system, MCF-7 human breast carcinoma cells organize and develop into microtissues that contain a luminal space, in stark contrast to the flat morphology of MCF-7 two-dimensional (2D) monolayer cultures. Following exposure to 1nM E2, expression of typical estro ...[more]