Ontology highlight
ABSTRACT:
SUBMITTER: Ahmad S
PROVIDER: S-EPMC4933879 | biostudies-literature | 2016 Jul
REPOSITORIES: biostudies-literature
Ahmad Shoeb S Pecqueur Ludovic L Dreier Birgit B Hamdane Djemel D Aumont-Nicaise Magali M Plückthun Andreas A Knossow Marcel M Gigant Benoît B
Scientific reports 20160706
Affinity maturation by random mutagenesis and selection is an established technique to make binding molecules more suitable for applications in biomedical research, diagnostics and therapy. Here we identified an unexpected novel mechanism of affinity increase upon in vitro evolution of a tubulin-specific designed ankyrin repeat protein (DARPin). Structural analysis indicated that in the progenitor DARPin the C-terminal capping repeat (C-cap) undergoes a 25° rotation to avoid a clash with tubulin ...[more]