Ontology highlight
ABSTRACT:
SUBMITTER: Bao J
PROVIDER: S-EPMC4935452 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Bao Ji J Wu Qiong Q Wang Yujia Y Li Yi Y Li Li L Chen Fei F Wu Xiujuan X Xie Mingjun M Bu Hong H
International journal of molecular medicine 20160614 2
In the present study, we aimed to determine whether the combination of aggregate culture and decellularized liver scaffolds (DLSs) promoted the hepatic differentiation of murine bone marrow-derived mesenchymal stem cells (BM-MSCs) into high yields of mature hepatocytes in vitro. Four culturing methods for differentiation [single cell (2D), spheroids (3D), 2D + DLS and 3D + DLS] were studied. To determine the differentiation stages of the MSCs, RT-qPCR of the hepatocyte genes, immunostaining of h ...[more]