Molecular characterization of exon 3 of caprine myostatin gene in Marwari goat.

ABSTRACT:

Aim

To estimate genetic variability in exon 3 of caprine myostatin gene in Marwari goats.

Materials and methods

A total of 120 blood samples from unrelated Marwari goats were randomly collected from different villages of Bikaner (Rajasthan), India. Genomic DNA was extracted from whole blood using blood DNA isolation kit (Himedia Ltd.) as per manufacturer's protocol. The quality of extracted genomic DNA was checked on 0.8% agarose gel. Specifically designed a primer set for caprine myostatin (MSTN) gene (Genebank accession no. DQ167575) was used to amplify the exon 3 region of MSTN gene in Marwari goat. The genetic variability in exon 3 of MSTN gene in Marwari goat was assessed on 8% polyacrylamide gel electrophoresis to detect single strand conformation polymorphism (SSCP) p

SUBMITTER: Khichar JP 

PROVIDER: S-EPMC4937062 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature