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SharpViSu: integrated analysis and segmentation of super-resolution microscopy data.


ABSTRACT:

Unlabelled

We introduce SharpViSu, an interactive open-source software with a graphical user interface, which allows performing processing steps for localization data in an integrated manner. This includes common features and new tools such as correction of chromatic aberrations, drift correction based on iterative cross-correlation calculations, selection of localization events, reconstruction of 2D and 3D datasets in different representations, estimation of resolution by Fourier ring correlation, clustering analysis based on Voronoi diagrams and Ripley's functions. SharpViSu is optimized to work with eventlist tables exported from most popular localization software. We show applications of these on single and double-labelled super-resolution data.

Availability and implementation

SharpViSu is available as open source code and as compiled stand-alone application under https://github.com/andronovl/SharpViSu

Contact

klaholz@igbmc.fr

Supplementary information

Supplementary data are available at Bioinformatics online.

SUBMITTER: Andronov L 

PROVIDER: S-EPMC4937188 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

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Publications

SharpViSu: integrated analysis and segmentation of super-resolution microscopy data.

Andronov Leonid L   Lutz Yves Y   Vonesch Jean-Luc JL   Klaholz Bruno P BP  

Bioinformatics (Oxford, England) 20160318 14


<h4>Unlabelled</h4>We introduce SharpViSu, an interactive open-source software with a graphical user interface, which allows performing processing steps for localization data in an integrated manner. This includes common features and new tools such as correction of chromatic aberrations, drift correction based on iterative cross-correlation calculations, selection of localization events, reconstruction of 2D and 3D datasets in different representations, estimation of resolution by Fourier ring c  ...[more]

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