Ontology highlight
ABSTRACT:
SUBMITTER: Pindyurin AV
PROVIDER: S-EPMC4937306 | biostudies-literature | 2016 Jul
REPOSITORIES: biostudies-literature
Nucleic acids research 20160321 12
Dam identification (DamID) is a powerful technique to generate genome-wide maps of chromatin protein binding. Due to its high sensitivity, it is particularly suited to study the genome interactions of chromatin proteins in small tissue samples in model organisms such as Drosophila Here, we report an intein-based approach to tune the expression level of Dam and Dam-fusion proteins in Drosophila by addition of a ligand to fly food. This helps to suppress possible toxic effects of Dam. In addition, ...[more]