Ontology highlight
ABSTRACT:
SUBMITTER: Quinet A
PROVIDER: S-EPMC4937316 | biostudies-literature | 2016 Jul
REPOSITORIES: biostudies-literature
Quinet Annabel A Martins Davi Jardim DJ Vessoni Alexandre Teixeira AT Biard Denis D Sarasin Alain A Stary Anne A Menck Carlos Frederico Martins CF
Nucleic acids research 20160419 12
Ultraviolet-induced 6-4 photoproducts (6-4PP) and cyclobutane pyrimidine dimers (CPD) can be tolerated by translesion DNA polymerases (TLS Pols) at stalled replication forks or by gap-filling. Here, we investigated the involvement of Polη, Rev1 and Rev3L (Polζ catalytic subunit) in the specific bypass of 6-4PP and CPD in repair-deficient XP-C human cells. We combined DNA fiber assay and novel methodologies for detection and quantification of single-stranded DNA (ssDNA) gaps on ongoing replicatio ...[more]