In Vitro Effects of the Endocrine Disruptor p,p'-DDT on Human Follitropin Receptor.

ABSTRACT:

Background

1-chloro-4-[2,2,2-trichloro-1-(4-chlorophenyl)ethyl]benzene (p,p'-DDT) is a persistent environmental endocrine disruptor (ED). Several studies have shown an association between p,p'-DDT exposure and reproductive abnormalities.

Objectives

To investigate the putative effects of p,p'-DDT on the human follitropin receptor (FSHR) function.

Methods and results

We used Chinese hamster ovary (CHO) cells stably expressing human FSHR to investigate the impact of p,p'-DDT on FSHR activity and its interaction with the receptor. At a concentration of 5 μM p,p'-DDT increased the maximum response of the FSHR to follitropin by 32 ± 7.45%. However, 5 μM p,p'-DDT decreased the basal activity and did not influence the maximal response of the closely related LH/hCG receptor to

SUBMITTER: Munier M 

PROVIDER: S-EPMC4937862 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature