Ontology highlight
ABSTRACT:
SUBMITTER: Borkent M
PROVIDER: S-EPMC4939549 | biostudies-literature | 2016 May
REPOSITORIES: biostudies-literature
Borkent Marti M Bennett Brian D BD Lackford Brad B Bar-Nur Ori O Brumbaugh Justin J Wang Li L Du Ying Y Fargo David C DC Apostolou Effie E Cheloufi Sihem S Maherali Nimet N Elledge Stephen J SJ Hu Guang G Hochedlinger Konrad K
Stem cell reports 20160303 5
The generation of induced pluripotent stem cells (iPSCs) from differentiated cells following forced expression of OCT4, KLF4, SOX2, and C-MYC (OKSM) is slow and inefficient, suggesting that transcription factors have to overcome somatic barriers that resist cell fate change. Here, we performed an unbiased serial shRNA enrichment screen to identify potent repressors of somatic cell reprogramming into iPSCs. This effort uncovered the protein modifier SUMO2 as one of the strongest roadblocks to iPS ...[more]