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Conjugating Prussian blue nanoparticles onto antigen-specific T cells as a combined nanoimmunotherapy.

ABSTRACT:

Aim

To engineer a novel nanoimmunotherapy comprising Prussian blue nanoparticles (PBNPs) conjugated to antigen-specific cytotoxic T lymphocytes (CTL), which leverages PBNPs for their photothermal therapy (PTT) capabilities and Epstein-Barr virus (EBV) antigen-specific CTL for their ability to traffic to and destroy EBV antigen-expressing target cells.

Materials & methods

PBNPs and CTL were independently biofunctionalized. Subsequently, PBNPs were conjugated onto CTL using avidin-biotin interactions. The resultant cell-nanoparticle construct (CTL:PBNPs) were analyzed for their physical, phenotypic and functional properties.

Results

Both PBNPs and CTL maintained their intrinsic physical, phenotypic and functional properties within the CTL:PBNPs.

Conclusion

This study highlights the potential of our CTL:PBNPs nanoimmunotherapy as a novel therapeutic for treating virus-associated malignancies such as EBV+ cancers.

SUBMITTER: Burga RA

PROVIDER: S-EPMC4941545 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

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Conjugating Prussian blue nanoparticles onto antigen-specific T cells as a combined nanoimmunotherapy.

Burga Rachel A RA Patel Shabnum S Bollard Catherine M CM Y Cruz Conrad Russell CR Fernandes Rohan R

Nanomedicine (London, England) 20160707 14

<h4>Aim</h4>To engineer a novel nanoimmunotherapy comprising Prussian blue nanoparticles (PBNPs) conjugated to antigen-specific cytotoxic T lymphocytes (CTL), which leverages PBNPs for their photothermal therapy (PTT) capabilities and Epstein-Barr virus (EBV) antigen-specific CTL for their ability to traffic to and destroy EBV antigen-expressing target cells.<h4>Materials & methods</h4>PBNPs and CTL were independently biofunctionalized. Subsequently, PBNPs were conjugated onto CTL using avidin-b ...[more]

PMID: 27389189

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