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Quantitative super-resolution imaging with qPAINT.

ABSTRACT: Counting molecules in complexes is challenging, even with super-resolution microscopy. Here, we use the programmable and specific binding of dye-labeled DNA probes to count integer numbers of targets. This method, called quantitative points accumulation in nanoscale topography (qPAINT), works independently of dye photophysics for robust counting with high precision and accuracy over a wide dynamic range. qPAINT was benchmarked on DNA nanostructures and demonstrated for cellular applications by quantifying proteins in situ and the number of single-molecule FISH probes bound to an mRNA target.

SUBMITTER: Jungmann R

PROVIDER: S-EPMC4941813 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

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Quantitative super-resolution imaging with qPAINT.

Jungmann Ralf R Avendaño Maier S MS Dai Mingjie M Woehrstein Johannes B JB Agasti Sarit S SS Feiger Zachary Z Rodal Avital A Yin Peng P

Nature methods 20160328 5

Counting molecules in complexes is challenging, even with super-resolution microscopy. Here, we use the programmable and specific binding of dye-labeled DNA probes to count integer numbers of targets. This method, called quantitative points accumulation in nanoscale topography (qPAINT), works independently of dye photophysics for robust counting with high precision and accuracy over a wide dynamic range. qPAINT was benchmarked on DNA nanostructures and demonstrated for cellular applications by q ...[more]

PMID: 27018580

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