Ontology highlight
ABSTRACT:
SUBMITTER: Liu Y
PROVIDER: S-EPMC4942795 | biostudies-literature | 2016 Jul
REPOSITORIES: biostudies-literature
Liu Yuchen Y Chen Zhicong Z He Anbang A Zhan Yonghao Y Li Jianfa J Liu Li L Wu Hanwei H Zhuang Chengle C Lin Junhao J Zhang Qiaoxia Q Huang Weiren W
Scientific reports 20160713
Recently CRISPR-Cas9 system has been reported to be capable of targeting a viral RNA, and this phenomenon thus raises an interesting question of whether Cas9 can also influence translation of cellular mRNAs. Here, we show that both natural and catalytically dead Cas9 can repress mRNA translation of cellular genes, and that only the first 14 nt in the 5' end of sgRNA is essential for this process. CRISPR-Cas9 can suppress the protein expression of an unintended target gene without affecting its D ...[more]