Dataset Information

Exercise duration-matched interval and continuous sprint cycling induce similar increases in AMPK phosphorylation, PGC-1? and VEGF mRNA expression in trained individuals.

ABSTRACT: The effects of low-volume interval and continuous 'all-out' cycling, matched for total exercise duration, on mitochondrial and angiogenic cell signalling was investigated in trained individuals.In a repeated measures design, 8 trained males ([Formula: see text], 57 ± 7 ml kg(-1) min(-1)) performed two cycling exercise protocols; interval (INT, 4 × 30 s maximal sprints interspersed by 4 min passive recovery) or continuous (CON, 2 min continuous maximal sprint). Muscle biopsies were obtained before, immediately after and 3 h post-exercise.Total work was 53 % greater (P = 0.01) in INT compared to CON (71.2 ± 7.3 vs. 46.3 ± 2.7 kJ, respectively). Phosphorylation of AMPK(Thr172) increased by a similar magnitude (P = 0.347) immediately post INT and CON (1.6 ± 0.2 and 1.3 ± 0.3 fold, respectively; P = 0.011), before returning to resting values at 3 h post-exercise. mRNA expression of PGC-1? (7.1 ± 2.1 vs. 5.5 ± 1.8 fold; P = 0.007), VEGF (3.5 ± 1.2 vs. 4.3 ± 1.8 fold; P = 0.02) and HIF-1? (2.0 ± 0.5 vs. 1.5 ± 0.3 fold; P = 0.04) increased at 3 h post-exercise in response to INT and CON, respectively; the magnitude of which were not different between protocols.Despite differences in total work done, low-volume INT and CON 'all-out' cycling, matched for exercise duration, provides a similar stimulus for the induction of mitochondrial and angiogenic cell signalling pathways in trained skeletal muscle.

SUBMITTER: Taylor CW

PROVIDER: S-EPMC4943987 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Exercise duration-matched interval and continuous sprint cycling induce similar increases in AMPK phosphorylation, PGC-1α and VEGF mRNA expression in trained individuals.

Taylor Conor W CW Ingham Stephen A SA Hunt Julie E A JE Martin Neil R W NR Pringle Jamie S M JS Ferguson Richard A RA

European journal of applied physiology 20160601 8

<h4>Purpose</h4>The effects of low-volume interval and continuous 'all-out' cycling, matched for total exercise duration, on mitochondrial and angiogenic cell signalling was investigated in trained individuals.<h4>Methods</h4>In a repeated measures design, 8 trained males ([Formula: see text], 57 ± 7 ml kg(-1) min(-1)) performed two cycling exercise protocols; interval (INT, 4 × 30 s maximal sprints interspersed by 4 min passive recovery) or continuous (CON, 2 min continuous maximal sprint). Mus ...[more]

PMID: 27251406

Similar Datasets

Project description:BackgroundMany individuals lose less weight than expected in response to exercise interventions when considering the increased energy expenditure of exercise (ExEE). This is due to energy compensation in response to ExEE, which may include increases in energy intake (EI) and decreases in non-exercise physical activity (NEPA). We examined the degree of energy compensation in healthy young men and women in response to interval training.MethodsData were examined from a prior study in which 24 participants (mean age, BMI, & VO2max = 28 yrs, 27.7 kg•m-2, and 32 mL?kg-1?min-1) completed either 4 weeks of sprint-interval training or high-intensity interval training. Energy compensation was calculated from changes in body composition (air displacement plethysmography) and exercise energy expenditure was calculated from mean heart rate based on the heart rate-VO2 relationship. Differences between high (? 100%) and low (< 100%) levels of energy compensation were assessed. Linear regressions were utilized to determine associations between energy compensation and ?VO2max, ?EI, ?NEPA, and ?resting metabolic rate.ResultsVery large individual differences in energy compensation were noted. In comparison to individuals with low levels of compensation, individuals with high levels of energy compensation gained fat mass, lost fat-free mass, and had lower change scores for VO2max and NEPA. Linear regression results indicated that lower levels of energy compensation were associated with increases in ?VO2max (p < 0.001) and ?NEPA (p < 0.001).ConclusionsConsiderable variation exists in response to short-term, low dose interval training. In agreement with prior work, increases in ?VO2max and ?NEPA were associated with lower energy compensation. Future studies should focus on identifying if a dose-response relationship for energy compensation exists in response to interval training, and what underlying mechanisms and participant traits contribute to the large variation between individuals.

Project description:The primary aim of the present study was to investigate how the fatigue induced through a repeat sprint protocol acutely affected different measures of neuromuscular performance. Recreationally trained basketball players (n = 25) volunteered to participate in the study, and performed three countermovement jumps (CMJ), as well as three drop jumps (DJ) prior to a fatiguing repeat sprint protocol. These procedures were repeated two minutes, and 15 minutes, following the protocol. Various force-time metrics were extracted from the jump tasks, and linear mixed models with subject ID as the random factor, and time as the fixed factor were used to investigate changes across the three time points. To account for the performance during the repeat sprint protocol, a second, two factor model was performed with time and repeat sprint ability (RSA) as the fixed factors. Study results indicated that the sample as a whole merely experienced fatigue-induced decreases in jump height from pre-repeat sprint ability protocol (pre-RSA) within the CMJ compared to two minutes post-repeat sprint ability protocol (post-RSA1) and 15 minutes post-repeat sprint ability protocol (post-RSA2), while jump height within the DJ was only significantly different from pre-RSA at post-RSA1. Further, despite the implementation of the fatiguing RSA protocol, over the course of the three time-points, participants seemed to perform the two jump tasks more efficiently, seen through significantly lower contraction times, greater eccentric (ECC) peak power, and greater ECC mean deceleration force within the CMJ following the RSA task. The two-factor model revealed that several significant time*RSA interactions were found for metrics such as ECC peak velocity and peak power in the CMJ, as well as reactive strength index in the DJ. This suggests that the level of RSA influenced changes across CMJ and DJ characteristics and should be accounted for when interpreting fatigue-induced changes in neuromuscular performance.

Project description:Improving performances in sprinting requires feedback on sprint parameters such as step length and step time. However, these parameters from the top speed interval (TSI) are difficult to collect in a competition setting. Recent advances in tracking technology allows to provide positional data with high spatio-temporal resolution. This pilot study, therefore, aims to automatically obtain general sprint parameters, parameters characterizing, and derived from TSI from raw speed. In addition, we propose a method for obtaining the intra-cyclic speed amplitude in TSI. We analyzed 32 100 m-sprints of 7 male and 9 female athletes (18.9 ± 2.8 years; 100 m PB 10.55-12.41 s, respectively, 12.18-13.31 s). Spatio-temporal data was collected with a radio-based position detection system (RedFIR, Fraunhofer Institute, Germany). A general velocity curve was fitted to the overall speed curve (vbase), TSI (upper quintile of vbase values) was determined and a cosine term was added to vbase within TSI (vcycle) to capture the cyclic nature of speed. This allowed to derive TSI parameters including TSI amplitude from the fitted parameters of the cosine term. Results showed good approximation for vbase (error: 5.0 ± 1.0%) and for vcycle (2.0 ± 1.0%). For validation we compared spatio-temporal TSI parameters to criterion values from laser measurement (speed) and optoelectric systems (step time and step length) showing acceptable RMSEs for mean speed (0.08 m/s), for step time (0.004 s), and for step length (0.03 m). Top speed interval amplitude showed a significant difference between males (mean: 1.41 m/s) and females (mean: 0.71 m/s) and correlations showed its independence from other sprint parameters. Gender comparisons for validation revealed the expected differences. This pilot study investigated the feasibility of estimating sprint parameters from high-quality tracking data. The proposed method is independent of the data source and allows to automatically obtain general sprint parameters and TSI parameters, including TSI amplitude assessed here for the first time in a competition-like setting.

Dataset Information

Exercise duration-matched interval and continuous sprint cycling induce similar increases in AMPK phosphorylation, PGC-1? and VEGF mRNA expression in trained individuals.

Publications

Exercise duration-matched interval and continuous sprint cycling induce similar increases in AMPK phosphorylation, PGC-1α and VEGF mRNA expression in trained individuals.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets