Ontology highlight
ABSTRACT:
SUBMITTER: Ghodke H
PROVIDER: S-EPMC4944719 | biostudies-literature | 2016 Jul
REPOSITORIES: biostudies-literature
Ghodke Harshad H Caldas Victor E A VE Punter Christiaan M CM van Oijen Antoine M AM Robinson Andrew A
Biophysical journal 20160620 1
Tagging of individual proteins with genetically encoded fluorescent proteins (FPs) has been used extensively to study localization and interactions in live cells. Recent developments in single-molecule localization microscopy have enabled the dynamic visualization of individual tagged proteins inside living cells. However, tagging proteins with FPs is not without problems: formation of insoluble aggregates and inhibition of native functions of the protein are well-known issues. Previously report ...[more]