Dataset Information

Next-generation sequencing methylation profiling of subjects with obesity identifies novel gene changes.

ABSTRACT:

Background

Obesity is a metabolic disease caused by environmental and genetic factors. However, the epigenetic mechanisms of obesity are incompletely understood. The aim of our study was to investigate the role of skeletal muscle DNA methylation in combination with transcriptomic changes in obesity.

Results

Muscle biopsies were obtained basally from lean (n?=?12; BMI?=?23.4?±?0.7 kg/m(2)) and obese (n?=?10; BMI?=?32.9?±?0.7 kg/m(2)) participants in combination with euglycemic-hyperinsulinemic clamps to assess insulin sensitivity. We performed reduced representation bisulfite sequencing (RRBS) next-generation methylation and microarray analyses on DNA and RNA isolated from vastus lateralis muscle biopsies. There were 13,130 differentially methylated cytosines (DMC; uncorrected P?ConclusionsThese results demonstrate that obesity alters the epigenome through DNA methylation and highlights novel transcriptomic changes in SORBS3 in skeletal muscle.

SUBMITTER: Day SE

PROVIDER: S-EPMC4950754 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Publications

Next-generation sequencing methylation profiling of subjects with obesity identifies novel gene changes.

Day Samantha E SE Coletta Richard L RL Kim Joon Young JY Campbell Latoya E LE Benjamin Tonya R TR Roust Lori R LR De Filippis Elena A EA Dinu Valentin V Shaibi Gabriel Q GQ Mandarino Lawrence J LJ Coletta Dawn K DK

Clinical epigenetics 20160718

<h4>Background</h4>Obesity is a metabolic disease caused by environmental and genetic factors. However, the epigenetic mechanisms of obesity are incompletely understood. The aim of our study was to investigate the role of skeletal muscle DNA methylation in combination with transcriptomic changes in obesity.<h4>Results</h4>Muscle biopsies were obtained basally from lean (n = 12; BMI = 23.4 ± 0.7 kg/m(2)) and obese (n = 10; BMI = 32.9 ± 0.7 kg/m(2)) participants in combination with euglycemic-hype ...[more]

PMID: 27437034

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Dataset Information

Next-generation sequencing methylation profiling of subjects with obesity identifies novel gene changes.

Background

Results

Publications

Next-generation sequencing methylation profiling of subjects with obesity identifies novel gene changes.

Similar Datasets

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