In vitro antioxidant, anti-diabetic and antilipemic potentials of quercetagetin extracted from marigold (Tagetes erecta L.) inflorescence residues.

ABSTRACT: Quercetagetin, the major flavonoid in marigold (Tagetes erecta L.) inflorescence residues was extracted and purified. The content of quercetagetin after the purification was 89.91?±?0.26 %. The in vitro antioxidant activity of quercetagetin and its potential in controlling diabetes mellitus and obesity were investigated and compared to quercetin and rutin. The 50 % inhibitory concentration (IC50) values of quercetagetin on scavenging 1, 1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azinobis-(3-ethylbenzothiazolin-6-sulfonic acid) (ABTS) and hydroxyl radicals were 27.12?±?1.31 ?mol/L, 12.16?±?0.56 ?mol/L and 1833.97?±?6.66 ?mol/L, respectively. The IC50 values of quercetagetin on ?-glucosidase, ?-amylase and pancreatic lipase were 180.11?±?3.68 ?mol/L, 137.71?±?3.55 ?mol/L and 2327.58?±?12.37 ?mol/L, respectively. These results indicated that quercetagetin exhibited strong in vitro antioxidant, anti-diabetic and antilipemic activities. Lineweaver-Burk plots analysis elucidated that quercetagetin inhibited ?-glucosidase and ?-amylase non-competitively, while its inhibition against pancreatic lipase was involved in a mixed-type pattern. Moreover, strong correlations were found between ABTS(·+)/DPPH(·) scavenging activities and lipase inhibitory activity (R (2)?>?0.90), as well as ·OH scavenging activity and ?-amylase inhibitory activity (R (2)?=?0.8967).

SUBMITTER: Wang W 

PROVIDER: S-EPMC4951414 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature