Unknown

Dataset Information

0

Functional Exploration of the Polysaccharide Lyase Family PL6.


ABSTRACT: Alginate, the main cell-wall polysaccharide of brown algae, is composed of two residues: mannuronic acid (M-residues) and, its C5-epimer, guluronic acid (G-residues). Alginate lyases define a class of enzymes that cleave the glycosidic bond of alginate by ?-elimination. They are classified according to their ability to recognize the distribution of M- and G-residues and are named M-, G- or MG-lyases. In the CAZy database, alginate lyases have been grouped by sequence similarity into seven distinct polysaccharide lyase families. The polysaccharide lyase family PL6 is subdivided into three subfamilies. Subfamily PL6_1 includes three biochemically characterized enzymes (two alginate lyases and one dermatan sulfatase lyase). No characterized enzymes have been described in the two other subfamilies (PL6_2 and PL6_3). To improve the prediction of polysaccharide-lyase activity in the PL6 family, we re-examined the classification of the PL6 family and biochemically characterized a set of enzymes reflecting the diversity of the protein sequences. Our results show that subfamily PL6_1 includes two dermatan sulfates lyases and several alginate lyases that have various substrate specificities and modes of action. In contrast, subfamilies PL6_2 and PL6_3 were found to contain only endo-poly-MG-lyases.

SUBMITTER: Mathieu S 

PROVIDER: S-EPMC4954714 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

altmetric image

Publications

Functional Exploration of the Polysaccharide Lyase Family PL6.

Mathieu Sophie S   Henrissat Bernard B   Labre Flavien F   Skjåk-Bræk Gudmund G   Helbert William W  

PloS one 20160720 7


Alginate, the main cell-wall polysaccharide of brown algae, is composed of two residues: mannuronic acid (M-residues) and, its C5-epimer, guluronic acid (G-residues). Alginate lyases define a class of enzymes that cleave the glycosidic bond of alginate by β-elimination. They are classified according to their ability to recognize the distribution of M- and G-residues and are named M-, G- or MG-lyases. In the CAZy database, alginate lyases have been grouped by sequence similarity into seven distin  ...[more]

Similar Datasets

| S-EPMC6223089 | biostudies-literature
| S-EPMC8377490 | biostudies-literature
| S-EPMC6879350 | biostudies-literature
| S-EPMC2612218 | biostudies-literature
| S-EPMC8174760 | biostudies-literature
| S-EPMC4882643 | biostudies-literature
| S-EPMC5377765 | biostudies-literature
| S-EPMC7460543 | biostudies-literature
| S-EPMC2790987 | biostudies-literature
| S-EPMC5313092 | biostudies-literature