The miRNAome of durum wheat: isolation and characterisation of conserved and novel microRNAs and their target genes.

ABSTRACT:

Background

The allotetraploid durum wheat [Triticum turgidum subsp. durum (Desf.) Husn.] is a highly economically important species especially in the Mediterranean basin. However, its genomics, transcriptomics and in particular microRNAome are still largely unknown.

Results

In the present work, two small RNA libraries from durum wheat Ciccio and Svevo cultivars were generated from different tissues at the late milk (Z77) developmental stage. A total of 167 conserved and 98 potential novel miRNAs were identified in the two libraries and interestingly, three novel miRNAs were found to be derived from ribosomal RNA. Putative target genes were predicted for conserved and novel miRNAs, the majority of which interact with nucleic acids, according to GO terms relative to molecular

SUBMITTER: De Paola D 

PROVIDER: S-EPMC4957382 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature