Role of transglutaminase 2 in liver injury via cross-linking and silencing of transcription factor Sp1.

ABSTRACT:

Background & aims

Despite high morbidity and mortality of alcoholic liver disease worldwide, the molecular mechanisms underlying alcohol-induced liver cell death are not fully understood. Transglutaminase 2 (TG2) is a cross-linking enzyme implicated in apoptosis. TG2 levels and activity are increased in association with various types of liver injury. However, how TG2 induces hepatic apoptosis is not known.

Methods

Human hepatic cells or primary hepatocytes from rats or TG2+/+ and TG2-/- mice were treated with ethanol. Mice were administered anti-Fas antibody or alcohol. Liver sections were prepared from patients with alcoholic steatohepatitis. Changes in TG2 levels, Sp1 cross-linking and its activities, expression of hepatocyte growth factor receptor, c-Met, and hepatic apop

SUBMITTER: Tatsukawa H 

PROVIDER: S-EPMC4960455 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature