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Construction of a tunable multi-enzyme-coordinate expression system for biosynthesis of chiral drug intermediates.

ABSTRACT: Systems that can regulate and coordinate the expression of multiple enzymes for metabolic regulation and synthesis of important drug intermediates are poorly explored. In this work, a strategy for constructing a tunable multi-enzyme-coordinate expression system for biosynthesis of chiral drug intermediates was developed and evaluated by connecting protein-protein expressions, regulating the strength of ribosome binding sites (RBS) and detecting the system capacity for producing chiral amino acid. Results demonstrated that the dual-enzyme system had good enantioselectivity, low cost, high stability, high conversion rate and approximately 100% substrate conversion. This study has paved a new way of exploring metabolic mechanism of functional genes and engineering whole cell-catalysts for synthesis of chiral ?-hydroxy acids or chiral amino acids.

SUBMITTER: Jiang W

PROVIDER: S-EPMC4960608 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

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Construction of a tunable multi-enzyme-coordinate expression system for biosynthesis of chiral drug intermediates.

Jiang Wei W Fang Baishan B

Scientific reports 20160726

Systems that can regulate and coordinate the expression of multiple enzymes for metabolic regulation and synthesis of important drug intermediates are poorly explored. In this work, a strategy for constructing a tunable multi-enzyme-coordinate expression system for biosynthesis of chiral drug intermediates was developed and evaluated by connecting protein-protein expressions, regulating the strength of ribosome binding sites (RBS) and detecting the system capacity for producing chiral amino acid ...[more]

PMID: 27456301

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