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Whole exome sequencing to estimate alloreactivity potential between donors and recipients in stem cell transplantation.


ABSTRACT: Whole exome sequencing (WES) was performed on stem cell transplant donor-recipient (D-R) pairs to determine the extent of potential antigenic variation at a molecular level. In a small cohort of D-R pairs, a high frequency of sequence variation was observed between the donor and recipient exomes independent of human leucocyte antigen (HLA) matching. Nonsynonymous, nonconservative single nucleotide polymorphisms were approximately twice as frequent in HLA-matched unrelated, compared with related D-R pairs. When mapped to individual chromosomes, these polymorphic nucleotides were uniformly distributed across the entire exome. In conclusion, WES reveals extensive nucleotide sequence variation in the exomes of HLA-matched donors and recipients.

SUBMITTER: Sampson JK 

PROVIDER: S-EPMC4964978 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature

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Whole exome sequencing to estimate alloreactivity potential between donors and recipients in stem cell transplantation.

Sampson Juliana K JK   Sheth Nihar U NU   Koparde Vishal N VN   Scalora Allison F AF   Serrano Myrna G MG   Lee Vladimir V   Roberts Catherine H CH   Jameson-Lee Max M   Ferreira-Gonzalez Andrea A   Manjili Masoud H MH   Buck Gregory A GA   Neale Michael C MC   Toor Amir A AA  

British journal of haematology 20140418 4


Whole exome sequencing (WES) was performed on stem cell transplant donor-recipient (D-R) pairs to determine the extent of potential antigenic variation at a molecular level. In a small cohort of D-R pairs, a high frequency of sequence variation was observed between the donor and recipient exomes independent of human leucocyte antigen (HLA) matching. Nonsynonymous, nonconservative single nucleotide polymorphisms were approximately twice as frequent in HLA-matched unrelated, compared with related  ...[more]

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