Ontology highlight
ABSTRACT:
SUBMITTER: Matsuda R
PROVIDER: S-EPMC4965299 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Matsuda Ryan R Kolli Venkata V Woods Megan M Dodds Eric D ED Hage David S DS
Analytical biochemistry 20160616
Sample pretreatment was optimized to obtain high sequence coverage for human serum albumin (HSA, 66.5 kDa) when using nano-electrospray ionization quadrupole time-of-flight mass spectrometry (nESI-Q-TOF-MS). Use of the final method with trypsin, Lys-C, and Glu-C digests gave a combined coverage of 98.8%. The addition of peptide fractionation resulted in 99.7% coverage. These results were comparable to those obtained previously with matrix-assisted laser desorption/ionization time-of-flight mass ...[more]