Ontology highlight
ABSTRACT:
SUBMITTER: Ali I
PROVIDER: S-EPMC4965572 | biostudies-literature | 2016 Jul
REPOSITORIES: biostudies-literature
Ali Ibraheem I Ramage Holly H Boehm Daniela D Dirk Lynnette M A LM Sakane Naoki N Hanada Kazuki K Pagans Sara S Kaehlcke Katrin K Aull Katherine K Weinberger Leor L Trievel Raymond R Schnoelzer Martina M Kamada Masafumi M Houtz Robert R Ott Melanie M
The Journal of biological chemistry 20160527 31
The HIV-1 transactivator protein Tat is a critical regulator of HIV transcription primarily enabling efficient elongation of viral transcripts. Its interactions with RNA and various host factors are regulated by ordered, transient post-translational modifications. Here, we report a novel Tat modification, monomethylation at lysine 71 (K71). We found that Lys-71 monomethylation (K71me) is catalyzed by KMT7, a methyltransferase that also targets lysine 51 (K51) in Tat. Using mass spectrometry, in ...[more]