Ontology highlight
ABSTRACT:
SUBMITTER: Auer GK
PROVIDER: S-EPMC4967499 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
Auer George K GK Lee Timothy K TK Rajendram Manohary M Cesar Spencer S Miguel Amanda A Huang Kerwyn Casey KC Weibel Douglas B DB
Cell systems 20160616 6
Bacteria must maintain mechanical integrity to withstand the large osmotic pressure differential across the cell membrane and wall. Although maintaining mechanical integrity is critical for proper cellular function, a fact exploited by prominent cell-wall-targeting antibiotics, the proteins that contribute to cellular mechanics remain unidentified. Here, we describe a high-throughput optical method for quantifying cell stiffness and apply this technique to a genome-wide collection of ∼4,000 Esch ...[more]