Ontology highlight
ABSTRACT:
SUBMITTER: Karim KM
PROVIDER: S-EPMC4967687 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
BioMed research international 20160718
A novel thermostable glucoamylase cDNA without starch binding domain (SBD) of Aspergillus flavus NSH9 was successfully identified, isolated, and overexpressed in Pichia pastoris GS115. The complete open reading frame of glucoamylase from Aspergillus flavus NSH9 was identified by employing PCR that encodes 493 amino acids lacking in the SBD. The first 17 amino acids were presumed to be a signal peptide. The cDNA was cloned into Pichia pastoris and the highest expression of recombinant glucoamylas ...[more]