A single amino acid substitution in a chitinase of the legume Medicago truncatula is sufficient to gain Nod-factor hydrolase activity.
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ABSTRACT:
SUBMITTER: Zhang LY
PROVIDER: S-EPMC4967823 | biostudies-literature | 2016 Jul
REPOSITORIES: biostudies-literature
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A single amino acid substitution in a chitinase of the legume Medicago truncatula is sufficient to gain Nod-factor hydrolase activity.
Zhang Lan-Yue LY Cai Jie J Li Ru-Jie RJ Liu Wei W Wagner Christian C Wong Kam-Bo KB Xie Zhi-Ping ZP Staehelin Christian C
Open biology 20160701 7
The symbiotic interaction between nitrogen-fixing rhizobia and legumes depends on lipo-chitooligosaccharidic Nod-factors (NFs). The NF hydrolase MtNFH1 of Medicago truncatula is a symbiotic enzyme that hydrolytically inactivates NFs with a C16 : 2 acyl chain produced by the microsymbiont Sinorhizobium meliloti 1021. MtNFH1 is related to class V chitinases (glycoside hydrolase family 18) but lacks chitinase activity. Here, we investigated the substrate specificity of MtNFH1-related proteins. MtCH ...[more]
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