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Trimer procyanidin oligomers contribute to the protective effects of cinnamon extracts on pancreatic ?-cells in vitro.

ABSTRACT:

Aim

Cinnamon extracts rich in procyanidin oligomers have shown to improve pancreatic ?-cell function in diabetic db/db mice. The aim of this study was to identify the active compounds in extracts from two species of cinnamon responsible for the pancreatic ?-cell protection in vitro.

Methods

Cinnamon extracts were prepared from Cinnamomum tamala (CT-E) and Cinnamomum cassia (CC-E). Six compounds procyanidin B2 (cpd1), (-)-epicatechin (cpd2), cinnamtannin B1 (cpd3), procyanidin C1 (cpd4), parameritannin A1 (cpd5) and cinnamtannin D1 (cpd6) were isolated from the extracts. INS-1 pancreatic ?-cells were exposed to palmitic acid (PA) or H2O2 to induce lipotoxicity and oxidative stress. Cell viability and apoptosis as well as ROS levels were assessed. Glucose-stimulated insulin secretion was examined in PA-treated ?-cells and murine islets.

Results

CT-E, CC-E as well as the compounds, except cpd5, did not cause cytotoxicity in the ?-cells up to the maximum dosage using in this experiment. CT-E and CC-E (12.5-50 ?g/mL) dose-dependently increased cell viability in both PA- and H2O2-treated ?-cells, and decreased ROS accumulation in H2O2-treated ?-cells. CT-E caused more prominent ?-cell protection than CC-E. Furthermore, CT-E (25 and 50 ?g/mL) dose-dependently increased glucose-stimulated insulin secretion in PA-treated ?-cells and murine islets, but CC-E had little effect. Among the 6 compounds, trimer procyanidins cpd3, cpd4 and cpd6 (12.5-50 ?mol/L) dose-dependently increased the cell viability and decreased ROS accumulation in H2O2-treated ?-cells. The trimer procyanidins also increased glucose-stimulated insulin secretion in PA-treated ?-cells.

Conclusion

Trimer procyanidins in the cinnamon extracts contribute to the pancreatic ?-cell protection, thus to the anti-diabetic activity.

SUBMITTER: Sun P

PROVIDER: S-EPMC4973380 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature

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Trimer procyanidin oligomers contribute to the protective effects of cinnamon extracts on pancreatic β-cells in vitro.

Sun Peng P Wang Ting T Chen Lu L Yu Bang-Wei BW Jia Qi Q Chen Kai-Xian KX Fan Hui-Min HM Li Yi-Ming YM Wang He-Yao HY

Acta pharmacologica Sinica 20160530 8

<h4>Aim</h4>Cinnamon extracts rich in procyanidin oligomers have shown to improve pancreatic β-cell function in diabetic db/db mice. The aim of this study was to identify the active compounds in extracts from two species of cinnamon responsible for the pancreatic β-cell protection in vitro.<h4>Methods</h4>Cinnamon extracts were prepared from Cinnamomum tamala (CT-E) and Cinnamomum cassia (CC-E). Six compounds procyanidin B2 (cpd1), (-)-epicatechin (cpd2), cinnamtannin B1 (cpd3), procyanidin C1 ( ...[more]

PMID: 27238208

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