Ontology highlight
ABSTRACT:
SUBMITTER: Riggle BA
PROVIDER: S-EPMC4976068 | biostudies-literature | 2015 Apr
REPOSITORIES: biostudies-literature
Journal of the American Chemical Society 20150420 16
Here we present a "smart" xenon-129 NMR biosensor that undergoes a peptide conformational change and labels cells in acidic environments. To a cryptophane host molecule with high Xe affinity, we conjugated a 30mer EALA-repeat peptide that is α-helical at pH 5.5 and disordered at pH 7.5. The (129)Xe NMR chemical shift at room temperature was strongly pH-dependent (Δδ = 3.4 ppm): δ = 64.2 ppm at pH 7.5 vs δ = 67.6 ppm at pH 5.5, where Trp(peptide)-cryptophane interactions were evidenced by Trp flu ...[more]