Effective High-Throughput Blood Pooling Strategy before DNA Extraction for Detection of Malaria in Low-Transmission Settings.
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ABSTRACT:
SUBMITTER: Nyunt MH
PROVIDER: S-EPMC4977795 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
Publications
Effective High-Throughput Blood Pooling Strategy before DNA Extraction for Detection of Malaria in Low-Transmission Settings.
Nyunt Myat Htut MH Kyaw Myat Phone MP Thant Kyaw Zin KZ Shein Thinzer T Han Soe Soe SS Zaw Ni Ni NN Han Jin-Hee JH Lee Seong-Kyun SK Muh Fauzi F Kim Jung-Yeon JY Cho Shin-Hyeong SH Lee Sang-Eun SE Yang Eun-Jeong EJ Chang Chulhun L CL Han Eun-Taek ET
The Korean journal of parasitology 20160630 3
In the era of (pre) elimination setting, the prevalence of malaria has been decreasing in most of the previously endemic areas. Therefore, effective cost- and time-saving validated pooling strategy is needed for detection of malaria in low transmission settings. In this study, optimal pooling numbers and lowest detection limit were assessed using known density samples prepared systematically, followed by genomic DNA extraction and nested PCR. Pooling strategy that composed of 10 samples in 1 poo ...[more]
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